Review



real time microscopic imaging instrument  (Sartorius AG)


Bioz Verified Symbol Sartorius AG is a verified supplier
Bioz Manufacturer Symbol Sartorius AG manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Sartorius AG real time microscopic imaging instrument
    Real Time Microscopic Imaging Instrument, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 15932 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/real+time+microscopic+imaging+instrument/Live+Cell+Analysis+Instruments/pmc12744928-103-22-28
    Average 99 stars, based on 15932 article reviews
    real time microscopic imaging instrument - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Concentration Assay:

    Article Title: Identification of non-oncology drugs with activity against inflammatory and triple-negative breast cancer cell lines
    Article Snippet: .. For the initial single concentration screening of the 48 drugs with SUM-149, we used a combination of real time imaging using a real-time microscopic imaging instrument (Incucyte S3, Sartorius) to monitor spheroid area and fluorescence from the CellTox Green cytotoxicity dye and subsequently, CTG was employed to detect viable cells by ATP detection. .. The cell lines were harvested, counted with a Vi-Cell XR Cell Viability Analyzer (Beckman Coulter) and then plated in 96-well plates (Sbio, Catalog # MS-9096UZ) at 5,000 cells/well in 100 μl/well complete growth media.

    Imaging:

    Article Title: Identification of non-oncology drugs with activity against inflammatory and triple-negative breast cancer cell lines
    Article Snippet: .. For the initial single concentration screening of the 48 drugs with SUM-149, we used a combination of real time imaging using a real-time microscopic imaging instrument (Incucyte S3, Sartorius) to monitor spheroid area and fluorescence from the CellTox Green cytotoxicity dye and subsequently, CTG was employed to detect viable cells by ATP detection. .. The cell lines were harvested, counted with a Vi-Cell XR Cell Viability Analyzer (Beckman Coulter) and then plated in 96-well plates (Sbio, Catalog # MS-9096UZ) at 5,000 cells/well in 100 μl/well complete growth media.

    Fluorescence:

    Article Title: Identification of non-oncology drugs with activity against inflammatory and triple-negative breast cancer cell lines
    Article Snippet: .. For the initial single concentration screening of the 48 drugs with SUM-149, we used a combination of real time imaging using a real-time microscopic imaging instrument (Incucyte S3, Sartorius) to monitor spheroid area and fluorescence from the CellTox Green cytotoxicity dye and subsequently, CTG was employed to detect viable cells by ATP detection. .. The cell lines were harvested, counted with a Vi-Cell XR Cell Viability Analyzer (Beckman Coulter) and then plated in 96-well plates (Sbio, Catalog # MS-9096UZ) at 5,000 cells/well in 100 μl/well complete growth media.

    CellTox Assay:

    Article Title: Identification of non-oncology drugs with activity against inflammatory and triple-negative breast cancer cell lines
    Article Snippet: .. For the initial single concentration screening of the 48 drugs with SUM-149, we used a combination of real time imaging using a real-time microscopic imaging instrument (Incucyte S3, Sartorius) to monitor spheroid area and fluorescence from the CellTox Green cytotoxicity dye and subsequently, CTG was employed to detect viable cells by ATP detection. .. The cell lines were harvested, counted with a Vi-Cell XR Cell Viability Analyzer (Beckman Coulter) and then plated in 96-well plates (Sbio, Catalog # MS-9096UZ) at 5,000 cells/well in 100 μl/well complete growth media.



    Similar Products

    real time microscopic imaging instrument  (Sartorius AG)
    99
    Sartorius AG real time microscopic imaging instrument
    Real Time Microscopic Imaging Instrument, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/real+time+microscopic+imaging+instrument/Live+Cell+Analysis+Instruments/pmc12744928-103-22-28
    Average 99 stars, based on 1 article reviews
    real time microscopic imaging instrument - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    incucyte zoom real time imaging microscope  (Sartorius AG)
    99
    Sartorius AG incucyte zoom real time imaging microscope
    (A) The protein expression levels of PAK4 were evaluated in SU.86.86, PANC-1, and PaTu-8988T cells with control shRNA or PAK4 knockdown. Vinculin was used as a protein-loading control. (B) A single-cell migration assay was used to evaluate the effect of PAK4 knockdown on the migration of SU.86.86, PANC-1, and PaTu-8988T cells. Scale bar, 100 μm (C) Cell migration was evaluated using a wound-healing assay. (D) A Matrigel-coated transwell assay was used to evaluate the effect of PAK4 knockdown on the invasive potential of SU.86.86 and PANC-1 cells. Scale bar, 100 μm (E) Cell displacement was analyzed by <t>IncuCyte</t> imaging in PANC-1 cells with shCTRL or shPAK4. (F) The sphere-forming potency of PANC-1 cells with PAK4 knockdown. Scale bar, 50 μm
    Incucyte Zoom Real Time Imaging Microscope, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/real+time+microscopic+imaging+instrument/Live+Cell+Analysis+Instruments/bio_rxiv__2024__05__22__594599-177-10-15
    Average 99 stars, based on 1 article reviews
    incucyte zoom real time imaging microscope - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) The protein expression levels of PAK4 were evaluated in SU.86.86, PANC-1, and PaTu-8988T cells with control shRNA or PAK4 knockdown. Vinculin was used as a protein-loading control. (B) A single-cell migration assay was used to evaluate the effect of PAK4 knockdown on the migration of SU.86.86, PANC-1, and PaTu-8988T cells. Scale bar, 100 μm (C) Cell migration was evaluated using a wound-healing assay. (D) A Matrigel-coated transwell assay was used to evaluate the effect of PAK4 knockdown on the invasive potential of SU.86.86 and PANC-1 cells. Scale bar, 100 μm (E) Cell displacement was analyzed by IncuCyte imaging in PANC-1 cells with shCTRL or shPAK4. (F) The sphere-forming potency of PANC-1 cells with PAK4 knockdown. Scale bar, 50 μm

    Journal: bioRxiv

    Article Title: Loss of p21-activated kinase 4 (PAK4) suppresses pancreatic tumor progression and metastasis through regulating E-cadherin

    doi: 10.1101/2024.05.22.594599

    Figure Lengend Snippet: (A) The protein expression levels of PAK4 were evaluated in SU.86.86, PANC-1, and PaTu-8988T cells with control shRNA or PAK4 knockdown. Vinculin was used as a protein-loading control. (B) A single-cell migration assay was used to evaluate the effect of PAK4 knockdown on the migration of SU.86.86, PANC-1, and PaTu-8988T cells. Scale bar, 100 μm (C) Cell migration was evaluated using a wound-healing assay. (D) A Matrigel-coated transwell assay was used to evaluate the effect of PAK4 knockdown on the invasive potential of SU.86.86 and PANC-1 cells. Scale bar, 100 μm (E) Cell displacement was analyzed by IncuCyte imaging in PANC-1 cells with shCTRL or shPAK4. (F) The sphere-forming potency of PANC-1 cells with PAK4 knockdown. Scale bar, 50 μm

    Article Snippet: To observe cell polarity of shCTRL and shPAK4 PANC-1 cells, IncuCyte Zoom real-time imaging microscope (Essen Bioscience) was used to image cells every 15 minutes for 24 hours.

    Techniques: Expressing, Control, shRNA, Knockdown, Cell Migration Assay, Migration, Wound Healing Assay, Transwell Assay, Imaging

    (A) The protein expression levels of PAK4 and E-cadherin were evaluated in control 293T and AsPC-1 cells and those transfected with a PAK4 overexpression plasmid. Vinculin was used as a protein-loading control. (B) A single-cell migration assay was used to evaluate the effect of PAK4 overexpression on the migration of AsPC-1 cells. Scale bar, 100 μm (C) A Matrigel-coated transwell assay was used to evaluate the effect of PAK4 overexpression on the invasive potential of AsPC-cells. Scale bar, 100 μm (D) A cell displacement assay, analyzed by IncuCyte imaging, was used to evaluate the effect of PAK4 overexpression on AsPC-1 cells. (E) Control AsPC-1 cells or those with PAK4 overexpression were incubated in serum-free medium for the indicated times. Activated Cdc42 and Rac1 were affinity-precipitated from whole cell lysate by incubating with GST-PBD-agarose. Bound Cdc42 and Rac1 were detected by western blot for Cdc42 and Rac1.

    Journal: bioRxiv

    Article Title: Loss of p21-activated kinase 4 (PAK4) suppresses pancreatic tumor progression and metastasis through regulating E-cadherin

    doi: 10.1101/2024.05.22.594599

    Figure Lengend Snippet: (A) The protein expression levels of PAK4 and E-cadherin were evaluated in control 293T and AsPC-1 cells and those transfected with a PAK4 overexpression plasmid. Vinculin was used as a protein-loading control. (B) A single-cell migration assay was used to evaluate the effect of PAK4 overexpression on the migration of AsPC-1 cells. Scale bar, 100 μm (C) A Matrigel-coated transwell assay was used to evaluate the effect of PAK4 overexpression on the invasive potential of AsPC-cells. Scale bar, 100 μm (D) A cell displacement assay, analyzed by IncuCyte imaging, was used to evaluate the effect of PAK4 overexpression on AsPC-1 cells. (E) Control AsPC-1 cells or those with PAK4 overexpression were incubated in serum-free medium for the indicated times. Activated Cdc42 and Rac1 were affinity-precipitated from whole cell lysate by incubating with GST-PBD-agarose. Bound Cdc42 and Rac1 were detected by western blot for Cdc42 and Rac1.

    Article Snippet: To observe cell polarity of shCTRL and shPAK4 PANC-1 cells, IncuCyte Zoom real-time imaging microscope (Essen Bioscience) was used to image cells every 15 minutes for 24 hours.

    Techniques: Expressing, Control, Transfection, Over Expression, Plasmid Preparation, Cell Migration Assay, Migration, Transwell Assay, Imaging, Incubation, Western Blot